Genome-wide mapping of DNase I hypersensitive sites in pineapple leaves

Pineapple [ Ananas comosus (L.) Merr.] is the most economically important crop possessing crassulacean acid metabolism (CAM) photosynthesis which has a higher water use efficiency by control of nocturnal opening and diurnal closure stomata. To provide novel insights into diel regulatory landscape in pineapple leaves, we performed genome-wide mapping DNase I hypersensitive sites (DHSs) leaves at day (2a.m.) night (10a.m.) using simplified DNase-seq method. As result, totally 33340 28753 DHSs were found green-tip tissue, 29597 40068 identified white-base tissue 2a.m. 10a.m., respectively. We observed that majority genes occupied less than two with length shorter 1 kb, promotor showed proximal trend to transcription start site (>77% within kb). In addition, more intergenic around factors or co-regulators (TFs/TCs) other functional genes, indicating complex contexts TFs/TCs. Through combined analysis preferential respectively 839 888 coordinately changed 10a.m. (AcG2 AcG10). Furthermore, AcG2-specific, AcG10-specific common accessible dissected from total photosynthetic DHSs, networks indicated dynamic regulations multiple cis -regulatory elements occurred preferentially expressed tissues. Interestingly, binding motifs several cycling TFs key CAM revealing circadian regulation expression. Our results chromatin during night. This will information assist deciphering photosynthesis.